Comparative mutagenicity of dimethylaminoazobenzene and analogues in Salmonella

Abstract
The mutagen and hepatocarcinogen 4-dimethylaminoazobenzene (DAB) and 3 of its structural analogs, N-methyl-5-phenylazoindoline, 4-N-pyrrolidinylazobenzene and 4''-ethyl-4-N-pyrrolidinylazobenzene were tested for mutagenicity in S. typhimurium TA1538. DAB was used as a standard and an S-9 mix containing 30% Aroclor-induced rat liver S-9 produced more mutagenic product than did an S-9 mix containing the standard 10% S-9. All chemicals tested were mutagenic and required S-9. Both pyrrolidine analogs produced similar responses to DAB, while the indoline was only weakly mutagenic.