Plant Cell Culture Using a Novel Bioreactor: The Magnetically Stabilized Fluidized Bed
- 1 November 1990
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 6 (6) , 452-457
- https://doi.org/10.1021/bp00006a008
Abstract
A novel bioreactor using magnetically stabilized fluidized bed (MSFB) technology has been developed that has certain advantages for cultivating cells continuously. In this system, the cells are protected from shear and are constrained to move through the fermenter in lock-step fashion by being immobilized in calcium alginate beads. The MSFB permits good mass transfer, minimizes particle collisions, and allows for the production of cells while maintaining a controlled cell residence time. Details of the experimental system are described. In addition, the experimental performance of an MSFB used to grow plant cells in batch mode is compared to the results obtained in shake flask culture.This publication has 14 references indexed in Scilit:
- Ethephon Enhancement of Secondary Metabolite Synthesis in Plant Cell CulturesBiotechnology Progress, 1988
- Commercializing Plant Tissue Culture Processes: Economics, Problems and ProspectsBiotechnology Progress, 1985
- Diffusion characteristics of substrates in Ca‐alginate gel beadsBiotechnology & Bioengineering, 1984
- Harvesting Profitable Products from Plant Tissue CultureNature Biotechnology, 1983
- The Relationship between Growth Rate, Differentiation and Alkaloid Accumulation in Cell CulturesJournal of Experimental Botany, 1983
- Studies on the production of digitalis cardenolides by plant tissue culture. Part IV. Effects of mineral salts, initial pH and precursors on digitoxin formation by shoot-forming cultures of Digitalis purpurea L. grown in liquid media.Agricultural and Biological Chemistry, 1983
- Immobilized plant cells for the production and transportation of natural productsFEBS Letters, 1979
- Fluidization: Hydrodynamic Stabilization with a Magnetic FieldScience, 1979
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962
- Lack of Bactericidal Effect of Mouse Serum on a Number of Common MicroorganismsScience, 1954