CALCIUM IONOPHORE A23187 INDUCES RELEASE OF CHEMOKINETIC AND AGGREGATING FACTORS FROM POLYMORPHO‐NUCLEAR LEUCOCYTES

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Abstract
1 Rat and human polymorphonuclear leucocytes (PMNs) when exposed to calcium ionophore A23187 10 μm release products which cause aggregation of rat PMNs and chemokinesis of human PMNs. 2 Aggregating and chemokinetic activities are rapidly generated; maximal release occurs after 4 min, and can be detected in dilutions of the supernatant of up to 1:1000. 3 Generation of aggregating and chemokinetic activities is inhibited by nordihydroguaiaretic acid 10−4 to 10−7 m, 5,8,11,14-eicosatetraynoic acid 10−4 and 10−5 m, BW 755C 10−4 m and benoxaprofen 10−4 m, all compounds known to inhibit lipoxygenase pathways of arachidonic acid (AA) metabolism. 4 Conventional non-steroidal anti-inflammatory agents, such as aspirin and indomethacin, inhibited little or not at all the generation of these activities. 5 We conclude that the aggregating and chemokinetic activities induced by A23187 represent generation of biologically active products of lipoxygenase pathways of AA metabolism.