Interactions of cadmium with yeast mannans.

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Abstract
The interactions of cadmium (Cd) ions with mannans from six species of yeast were studied using equilibrium dialysis. Among the six mannans examined, the binding capacity of the mannan of Saccharomyces carlsbergensis was the greatest, at about 146 .mu.mol/g mannan. For the other mannans, the amounts of bound metal ions ranged from 20-46 .mu.mol/g mannan. However, the dissociation constants of Cd-binding to each mannan were very siilar, at about 0.5 .times. 10-3 M. Scatchard plot analysis indicted that the mannans had a single type of binding sites. Pronase digestion of the mannans decreased their total nitrogen, accompanied with significant losses of Cd-binding. These results suggest that the carbohydrate moieties of the mannans were not positively involved in the Cd-binding of the yeast cell walls.

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