Derangement of regulation of protein degradation in transforming fibroblasts
Open Access
- 1 February 1982
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 2 (2) , 107-114
- https://doi.org/10.1007/bf01116176
Abstract
Changes in endogenous protein degradation of stable proteins with growth state have been observed in a number of normal cell lines. Increases in degradation of between 15% and 40% occurred in 10 cell lines at confluence. No such regulation of proteolysis was observed in 4 cell lines [2 lines of mouse fibroblast 3T3 cells, 3T6 cells and Chinese hamster ovary, CHO-K1 cells] which were clonogenic in soft agar. This derangement of regulation apparently coincides with the transformation of cells. Regulation disappears in spontaneously transforming fibroblasts and is decreased by the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate. Regulation reappeared in a growth control revertant of a transformed (3T3) line. [Other cell lines studied include human fibroblasts, baby hamster kidney BHK21c13 cells, African green monkey kidney BSC-1 cells and human embryonic lung MRC-5 cells.].This publication has 18 references indexed in Scilit:
- The effect of protein degradation on cellular growth characteristicsJournal of Cellular Physiology, 1978
- Effect of the growth state on protein turnover in two lines of cultured BHK cellsJournal of Cellular Physiology, 1977
- Intracellular protein degradation in growing, in density‐inhibited, and in serum‐restricted fibroblast culturesJournal of Cellular Physiology, 1977
- Protein metabolism during growth of vero cellsJournal of Cellular Physiology, 1977
- Effect of medium composition on protein degradation and DNA synthesis in rat embryo fibroblasts.Proceedings of the National Academy of Sciences, 1977
- Reduced rates of proteolysis in transformed cellsNature, 1977
- Effect of the growth state on protein turnover in L cellsExperimental Cell Research, 1976
- Studies on the turnover of plasma membranes in cultured mammalian cells.: II. Rates of synthesis and degradation of plasma membrane proteins and carbohydratesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- Fluctuations of DNA-dependent RNA polymerase and synthesis of macromolecules during the growth cycle of Novikoff rat hepatoma cells in suspension cultureJournal of Cellular Physiology, 1969
- QUANTITATIVE STUDIES OF THE GROWTH OF MOUSE EMBRYO CELLS IN CULTURE AND THEIR DEVELOPMENT INTO ESTABLISHED LINESThe Journal of cell biology, 1963