UV-induced photoproducts in human skin explants analysed by TLC and HPLC-radioactivity detection
- 1 December 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 16 (12) , 3015-3019
- https://doi.org/10.1093/carcin/16.12.3015
Abstract
The objective of this study was to apply the 32P-postlabelling method for the detection of UV-induced dipyrimidine photoadducts in human skin explants. [32P]TLC and [32P]HPLC analysis were used. These techniques allow detection of different combinations of cyclobutane dimers and 6–4 photoproducts in human skin at low doses of irradiation. Detection limit of the present method for some dinucleotide adducts is 7 normal nucleotides. Reaction of photochemical reversion of the cyclobutane dimers was performed to confirm the nature of the products detected in human skin explants.Keywords
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