Decondensation of mouse sperm chromatin in cell‐free extracts: A micromethod
- 1 November 1990
- journal article
- research article
- Published by Wiley in Molecular Reproduction and Development
- Vol. 27 (3) , 244-248
- https://doi.org/10.1002/mrd.1080270310
Abstract
A micromethod is presented which makes possible the analysis of mouse sperm nucleus decondensation in vitro using very small volumes of cytoplasmic preparations, even smaller than 1μl. We show that cell‐free extracts obtained from interphase HeLa cells as well as lysates from mouse eggs and embryos can sustain early stages of mouse sperm nucleus transformation, provided the sperm nuclear envelope is damaged or removed.Keywords
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