Synthesis and physical characterization of the self-complementary, alternating pyrimidine/purine hexanucleotide d[CGTACG]
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (20) , 6265-6271
- https://doi.org/10.1093/nar/10.20.6265
Abstract
The hexanucleotide d(CGTACG) has been synthesized by a phosphotriester method in which, for the first time, an 0-6 protected deoxyguanosine derivative has been used to avoid side reactions of the guanine residues. Conformational analysis by circular dichroism shows that d(CGTACG) maintains a B form under conditions (5 M NaCl) where the all C/G hexanucleotide d(CG)3 adopts a Z form, even though d(CGTACG) is a fully alternating pyrimidine/purine molecule. The ΔH for the helix-to-coil transition has been measured.Keywords
This publication has 12 references indexed in Scilit:
- Structure of a B-DNA dodecamer: conformation and dynamics.Proceedings of the National Academy of Sciences, 1981
- Left-handed DNA in restriction fragments and a recombinant plasmidNature, 1981
- Left-Handed Double Helical DNA: Variations in the Backbone ConformationScience, 1981
- Synthesis of oligodeoxyribonucleotides on silica gel supportNucleic Acids Research, 1981
- Simplifications in the synthesis of short oligonucleotide blocksNucleic Acids Research, 1980
- Chemical synthesis of a tridecanucleoside dodecaphosphate sequence of SV40 DNANucleic Acids Research, 1980
- Molecular structure of a left-handed double helical DNA fragment at atomic resolutionNature, 1979
- Chemical synthesis of a self-complementary octanucleotide, dG-G-T-T-A-A-C-C by a modified triester methodNucleic Acids Research, 1978
- Salt-induced co-operative conformational change of a synthetic DNA: Equilibrium and kinetic studies with poly(dG-dC)Journal of Molecular Biology, 1972
- Oligonucleotide interactions. III. Circular dichroism studies of the conformation of deoxyoligonucleolidesBiopolymers, 1970