A potent fungus for lipoxygenase-like enzyme production was isolated from soil during a screening test by the method of surface culture with soybean oil. The fungus was identified as Fusarium oxysporum. The production of a lipoxygenase-like enzyme was also seen from all other Fusarium sp. tested. The enzyme was produced intracellularly. A starting pH above 8, use of soybean oil as the carbon source and of soybean flour as the nitrogen source and low oxygen tension were necessary conditions for the optimal formation of the enzyme. Hydroperoxide produced by Fusarium lipoxygenase was observed in thin-layer chromato-graphy to coincide in Rf value with that produced by soybean lipoxygenase. Co2+ stabilized the enzyme. Optimal pH for the crude enzyme was 6 or 10. In the pH range of 12_??_13 the activity increased as pH rose. The enzyme showed higher specificity for linoleate.