isoCitric dehydrogenase and glutamic acid synthesis in animal tissues

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Abstract
From heart muscle an isocitric apodehydrogenase was prepared which catalysed the reaction isocitric acid + codehydrogenase II [long dash] [alpha]-keto-[beta]-carboxylic glutaric acid + dihydro-codehydrogenase II. The ketoacid decomposed spontaneously giving [alpha]-keto-glutaric acid and CO2. Codehydrogenase I was inactive in that system. The system did not react unless Mn++ or Mg++ was present, Mn++ being more active than Mg++. The apodehydrogenase was found in all animal tissues, the highest concs. being present in heart, liver and kidney. Iodoacetic acid and pyrophosphate were strong inhibitors. The reaction products, [alpha]-ketoglutaric acid and CoH2II, were utilized in animal tissues in presence of NH3, which combined with ketoglutaric acid to form iminoglutaric acid. The latter compound in the presence of glutamic apodehydrogenase was then reduced by the C0H2II, to yield glutamic acid.

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