CALCIUM MOVEMENTS IN BRAIN SLICES IN LOW SODIUM OR CALCIUM MEDIA

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Abstract
Abstract— Movement of 45Ca into and out of cerebral slices in vitro was altered by incubation in Na‐free medium or in the presence of ouabain. 45Ca uptake into the ‘non‐inulin’ space was more than doubled in slices that were losing Na into Na‐deficient media. Replacement of Na in the incubation medium by choline chloride reduced the efflux of 45Ca into Ca‐free medium. In the presence of ouabain (10‐4M), the rate of efflux was also reduced. NaCN or NaCN plus ethacrynic acid increased the rate of 45Ca efflux. The data are compatible with a sodium‐calcium exchange mechanism in cerebral slices.