The apoE polymorphism studied by two‐dimensional, high‐resolution gel electrophoresis of serum

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Abstract
The apoE polymorphism of human very low density lipoprotein (VLDL) was studied by the 2-dimensional high-resolution gel electrophoresis technique of O''Farrell, which combines isoelectric focusing and SDS-polyacrylamide gel electrophoresis. With whole serum, 6 major patterns and 2 sub-variants of apoE were observed. A series of twin pairs (21 monozygotic (MZ) and 13 dizygotic (DZ) pairs) as well as unrelated people were analyzed. Both members of MZ pairs always exhibited the same pattern, whereas DZ twins were often discordant. The patterns observed would be consistent with the hypothesis that 3 apoE isopeptides are coded for by 3 different alleles at 1 single locus. All 3 postulated homozygous patterns, namely apoE-II, apoE-III and apoE-IV as well as the 3 heterozygous patterns apoE-II,III; apoE-III,IV and apoE-II,IV were seen.