An efficient method of selecting photoautotrophic cells from cultured heterogeneous cells
- 1 August 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 21 (5) , 929-932
- https://doi.org/10.1093/oxfordjournals.pcp.a076067
Abstract
A new and efficient method was demonstrated for the establishment of photoautotrophic cultures of plant cells. Leaf segments of Atropa belladonna, Datura stramonium and Hyoscyamus niger were inoculated on sugar-free Linsmaier-Skoog agar medium then aerated with 1% CO2 enriched air under 3,000 to 5,000 lux of illumination. Under these regulated conditions we could select photoautotrophic green cells efficiently, and these cells subsequently have grown well under photoautotrophic conditions.Keywords
This publication has 1 reference indexed in Scilit:
- Organic Growth Factor Requirements of Tobacco Tissue CulturesPhysiologia Plantarum, 1965