Functional Requirement of a Site-Specific Ribose Methylation in Ribosomal RNA
- 17 December 1993
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 262 (5141) , 1886-1889
- https://doi.org/10.1126/science.8266080
Abstract
The product of the PET56 nuclear gene of Saccharomyces cerevisiae was shown to be required for ribose methylation at a universally conserved nucleotide in the peptidyl transferase center of the mitochondrial large ribosomal RNA (21S rRNA). Cells reduced in this activity were deficient in formation of functional large subunits of the mitochondrial ribosome. The purified Pet56 protein catalyzed the site-specific formation of 2'-O-methylguanosine on in vitro transcripts of both mitochondrial 21S rRNA and Escherichia coli 23S rRNA. These results provide evidence for an essential modified nucleotide in rRNA.Keywords
This publication has 31 references indexed in Scilit:
- Methylation sites in Escherichia coli ribosomal RNA: Localization and identification of four new sites of methylation in 23 S rRNABiochemistry, 1992
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Recovery of Soluble, Biologically Active Recombinant Proteins from Total Bacterial Lysates Using Ion Exchange ResinBio/Technology, 1988
- Reassembly of active 30S ribosomal subunits with an unmethylated in vitro transcribed 16S rRNABiochemistry and Cell Biology, 1987
- Isolation and characterization of a nucleolar 2'-O-methyltransferase from Ehrlich ascites tumor cellsBiochemistry, 1987
- The Mitochondrial Genotype Can Influence Nuclear Gene Expression in YeastScience, 1987
- Modification of mitochondrial ribosomal RNA from hamster cells: The presence of GmG and late-methylated UmGmU in the large subunit (17 S) RNAJournal of Molecular Biology, 1978
- Minimal post-transcriptional modification of yeast mitochondrial ribosomal RNAJournal of Molecular Biology, 1975
- Physical and genetic organization of petite and grande yeast mitochondrial DNA: IV.In vivo Transcription Products of Mitochondrial DNA and Localization of 23 S Ribosomal RNA in Petite Mutants of Saccharomyces cerevisiaeJournal of Molecular Biology, 1974
- The methylated nucleotide sequences in HeLa cell ribosomal RNA and its precursorsJournal of Molecular Biology, 1974