Matrix metalloproteinase 9 level predicts optimal collagen deposition during early wound repair in humans

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Abstract
Background: This study examined two matrix metalloproteinases (MMPs) and their association with deposition of collagen during wound repair. Methods: In 15 hernia wounds, wound fluid was collected using two Cellstick devices, which were implanted adjacent to two expanded polytetrafluoroethylene (ePTFE) tubes. MMP-2 and MMP-9 levels were analysed 24 and 48 h after operation using specific enzyme-linked immunosorbent assays, and collagen deposition in the ePTFE tubes was measured 10 days after operation as hydroxyproline. Results: The levels of both MMPs in wound fluid were increased compared with those of control serum, although MMP-9 decreased (P < 0·01) in the wounds 24–48 h after operation. The MMP-9 level at 24 h correlated inversely and specifically to deposited collagen measured as hydroxyproline (rS = −0·80, P < 0·01). Conclusion: These results indicate that the level of MMP-9 in the early inflammatory phase can predict the amount of collagen deposited later in the wound healing process.