A Method for the Preparation of Tsutsugamushi (Scrub Typhus) Antigen from Infected Yolk Sacs
- 1 January 1946
- journal article
- research article
- Published by JSTOR in Public Health Reports (1896-1970)
- Vol. 61 (22) , 778-781
- https://doi.org/10.2307/4585689
Abstract
A method is described for the prepn. of antigen from yolk-sacs infected with Rickettsia orientalis. The method consists of defatting the yolk-sacs by di -ethyl ether in the cold before grinding and addition of diluent. This procedure allows the prepn. of a crude tissue suspension rather than the usual emulsion and it is susceptible to further purification and concentration by fractionation with either (NH4)2SO4 or ethanol.Keywords
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