Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
- 1 August 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (8) , 3755-3759
- https://doi.org/10.1073/pnas.76.8.3755
Abstract
A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5''-phosphotransferase, EC 2.7.1.21) gene was cloned in E. coli. A recombinant plasmid, pFG5, was analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse [fibroblast] L cells. The TK coding sequence in the cloned fragment was localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, was constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.This publication has 22 references indexed in Scilit:
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