IDENTIFICATION OF A LESS IMMUNOREACTIVE FORM OF PROLACTIN IN THE RAT PITUITARY

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Abstract
Disc electrophoretic analysis of prolactin (PRL) in the rat pituitary is usually performed using a 7.5% concentration of acrylamide gel. Re-electrophoresis of the eluate of the PRL band from such a gel on polyacrylamides of serially increasing concentrations revealed that.the PRL band of 7.5% gel actually contains at least two additional proteins. One of these, whose apparent mol. wt. is 31,000, has significant pigeon crop-sac stimulating activity but very l i t t l e or no cross-reactivity in a RIA for rat PRL. It also has a rapid turnover r a t e in short-term in v i t r o incubations. This newly-found protein seems to be a nonimmunoreactive molecular variant of rat PRL.