Lipogenesis in isolated human sebaceous glands

Lipogenesis in isolated human sebaceous glands from [U-¹⁴C]glucose, [U-¹⁴C]leucine, [U-¹⁴C]isoleucine, and [U-¹⁴C]valine has been determined by thin-layer chromatography. Total lipogenesis from 2 [U-¹⁴C]glucose was 114.8 ± 22.3 per h (mean ± SE), with 53.8% being incorporated into triglycerides, 20.2% into squalene, 12.8% into phospholipids, 2.1% into cholesterol and 7.1% into wax monoester and cholesterol ester and 5% into di- and monoglycerides and free fatty acids. Total lipogenesis from 2 [U-¹⁴C]leucine, 2 mmo [U-¹⁴C]isoleucine, and 2 [U-¹⁴C]valine in the presence of 2 glucose was 26, 29 and 9%, respectively, of that seen with 2 glucose alone. The pattern of ¹⁴C distribution in the various lipid classes from the three U-¹⁴C-labelled branched-chain amino acids was not significantly different from that seen with [U-¹⁴C]glucose.