Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4 Å resolution
- 1 February 1997
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 4 (2) , 153-157
- https://doi.org/10.1038/nsb0297-153
Abstract
We solved the crystal structure of the homotetrameric single-stranded DNA binding (SSB) protein from human mitochondria at a resoluntion of 2.4 Å. The tetramer is formed by two dimers interacting head-to-head and shows D2 symmetry. Sequence-related tetrameric SSB proteins occur in prokaryotes and eukaryotic mitochondria; this is the first report of an atomic resolution structure of this type of protein. Using biochemical data and analysis of sequence homologies, we were able to correlate the functional properties with structure. We propose that ssDNA wraps around the tetrameric HsmtSSB protein through electropositive channels guided by flexible loops.Keywords
This publication has 20 references indexed in Scilit:
- Crystallization and Low Temperature Diffraction Studies of the DNA Binding Domain of the Single-stranded DNA Binding Protein from Escherichia coliJournal of Molecular Biology, 1994
- ESCHERICHIA COLI SINGLE-STRANDED DNA-BINDING PROTEIN: Multiple DNA-Binding Modes and CooperativitiesAnnual Review of Biochemistry, 1994
- Single‐stranded‐DNA‐binding proteins from human mitochondria and Escherichia coli have analogous physicochemical propertiesEuropean Journal of Biochemistry, 1994
- Escherichia coli Single-Stranded DNA-Binding Proteins: Multiple DNA-Binding Modes and CooperativitiesAnnual Review of Biochemistry, 1994
- MOLSCRIPT: a program to produce both detailed and schematic plots of protein structuresJournal of Applied Crystallography, 1991
- Improved methods for building protein models in electron density maps and the location of errors in these modelsActa Crystallographica Section A Foundations of Crystallography, 1991
- Amino acid 55 plays a central role in tetramerization and function of Escherichia coli single‐stranded DNA binding proteinEuropean Journal of Biochemistry, 1991
- Kinetics of binding of single-stranded DNA binding protein from Escherichia coli to single-stranded nucleic acidsBiochemistry, 1990
- Single-Stranded DNA Binding Proteins Required for DNA ReplicationAnnual Review of Biochemistry, 1986
- Novel crystal forms of a proteolytic core of the single‐stranded DNA‐binding protein (SSB) from E. coliFEBS Letters, 1984