Intraperitoneal Transplantation of Isologous Mesothelial Cells for Prevention of Adhesions

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Abstract
To study the effect of transplantation of mesothelial cells on the formation of adhesions after peritoneal abrasion. Animal study. Teaching hospital, Germany. 30 isologous eight-week old Lewis rats to allow for harvesting of the greater omentum from a separate group. The first group (n = 10) served as mesothelial cell donors. The other animals had laparotomy and induction of adhesions by standardised abrasion of the peritoneum. The trial group (n = 10) were given a suspension of 10(6) mesothelial cells/100 g body weight intraperitoneally and the control group (n = 10) an equal volume of culture medium. After 10 days the animals were killed. Measurements of the areas of adhesions by computer aided morphometry. The trial group developed a mean (SD) adhesion area of 122.7 (176.7) mm2, and the controls 310.5 (179.1) mm2. The corresponding medians (range) were 51.2 (0-547.1) and 274.3 (100.6-575.4). Transplantation of mesothelial cells resulted in a significant reduction in adhesion formation (Wilcoxon test, p<0.01). Intraperitoneal transplantation of mesothelial cells is an effective way of reducing the formation of adhesions.

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