Quantitative aspects of endocytic activity in lipid‐mediated transfections
- 9 April 2005
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 579 (12) , 2635-2642
- https://doi.org/10.1016/j.febslet.2005.03.083
Abstract
Variation in transfection efficiency observed in different cell-types is poorly understood. To investigate the influence of endocytic activity on lipid-mediated transfections, we have monitored both the processes in 12 different cell-types. The endocytic activity shows a strong positive correlation (P70% compared to controls. Our study based on several cell-types demonstrates for the first time that quantitative aspects of endocytosis have decisive influence on the overall process of lipid-mediated transgene expressionKeywords
This publication has 42 references indexed in Scilit:
- Synergy between cationic lipid and co-lipid determines the macroscopic structure and transfection activity of lipoplexesNucleic Acids Research, 2002
- Design, Synthesis, and Transfection Biology of Novel Cationic Glycolipids for Use in Liposomal Gene DeliveryJournal of Medicinal Chemistry, 2001
- Interplay in lipoplexes between type of pDNA promoter and lipid composition determines transfection efficiency of human growth hormone in NIH3T3 cells in cultureBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2001
- Plasmid DNA size does not affect the physicochemical properties of lipoplexes but modulates gene transfer efficiencyNucleic Acids Research, 1999
- Novel Series of Non-Glycerol-Based Cationic Transfection Lipids for Use in Liposomal Gene DeliveryJournal of Medicinal Chemistry, 1999
- Biological barriers to cellular delivery of lipid-based DNA carriersAdvanced Drug Delivery Reviews, 1999
- Synthesis and Characterization of Aromatic Ring-Based Cationic Lipids for Gene DeliveryIn VitroandIn VivoJournal of Drug Targeting, 1999
- Cationic Liposomes for Gene TherapyAngewandte Chemie International Edition in English, 1998
- Cellular and Molecular Barriers to Gene Transfer by a Cationic LipidJournal of Biological Chemistry, 1995
- A novel cationic liposome reagent for efficient transfection of mammalian cellsBiochemical and Biophysical Research Communications, 1991