An AutoAnalyzer Procedure for Serum Iron and Total Iron-Binding Capacity, with Use of Ferrozine
Open Access
- 1 September 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 17 (9) , 950-953
- https://doi.org/10.1093/clinchem/17.9.950
Abstract
An AutoAnalyzer method has been developed in which the recently introduced reagent for iron, 3-(2-pyridyl)-5,6-bis(4-phenylsulfonic acid)-1,2,4-triazine ("ferrozine"), is used. With the Technicon colorimeter or Gilford 300-N spectrophotometer in use, sampling rates may be 30/h or 50/h, respectively. Sample volume is 0.8 ml, and measurements are made at 560 nm. A procedure for measuring total iron-binding capacity is also described.Keywords
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