A Comparison of Adhesives Useable in the Cold for the Preparation of Cryostat Sections
- 1 January 1971
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 46 (4) , 167-169
- https://doi.org/10.3109/10520297109067848
Abstract
A variety of adhesives were used at —20 C to attach cryostat sections to cold slides and to allow fixation in the cryostat, thus preventing thawing artefacts. Of a range of adhesives readily available, a natural rubber solution, Romac C33, was found to give optimal results when used at this temperature. By this method histological, histochemical and immunofluorescent techniques could be successfully applied.Keywords
This publication has 4 references indexed in Scilit:
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- Freezing in an Isopentane-Liquid Nitrogen Mixture and Storage in 2-Octanol: Technical Improvements for ImmunofluorescenceStain Technology, 1966
- The Mounting of Carbowax Sections with Lighter-Fluid and Rubber CementStain Technology, 1961
- Cryostat Techniques: Methods for Improving Conservation and Sectioning of TissueStain Technology, 1960