Minichromosomes derived from the B chromosome of maize
- 1 March 2005
- journal article
- nuclear and-chromosome-organization
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 109 (1-3) , 156-165
- https://doi.org/10.1159/000082395
Abstract
Fourteen minichromosomes derived from the B chromosome of maize are described. The centromeric region of the B chromosome contains a specific repetitive DNA element called the B repeat. This sequence was used to determine the transmission frequency of the different types of minichromosomes over several generations via Southern blot analysis at each generation. In general, the minichromosomes have transmission rates below the theoretical 50% frequency of a univalent chromosome. The gross structure of each minichromosome was determined using fluorescence in situ hybridization (FISH) on root tip chromosome spreads. The presence of the B centromeric repeat and of the adjacent heterochromatic knob sequences was determined for each minichromosome. In two cases, the amount of the centromeric knob repeat is increased relative to the progenitor chromosome. Other isolates have reduced or undetectable levels of the knob sequence. Potential uses of the minichromosomes are discussed.Keywords
This publication has 30 references indexed in Scilit:
- Insertion of a Telomere Repeat Sequence into a Mammalian Gene Causes Chromosome InstabilityMolecular and Cellular Biology, 2001
- Construction of YAC–based mammalian artificial chromosomesNature Biotechnology, 1998
- Formation of de novo centromeres and construction of first-generation human artificial microchromosomesNature Genetics, 1997
- The molecular identification of the midget chromosome from the rye genomeGenome, 1994
- Origin and molecular structure of a midget chromosome in a common wheat carrying rye cytoplasmChromosoma, 1992
- Targeted breakage of a human chromosome mediated by cloned human telomeric DNANature Genetics, 1992
- Meiotic behavior of a tiny fragment chromosome that carries a transposed centromereGenome, 1987
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- THE B CHROMOSOME OF CORNAnnual Review of Genetics, 1978
- Misdivision and the genetics of the centromereJournal of Genetics, 1939