Alteration by mutation of the control by oxygen of the nar operon in Escherichia coli
- 1 November 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 205 (2) , 349-352
- https://doi.org/10.1007/bf00430449
Abstract
A nar-lac operon fusion was used to isolate a mutant in which the expression of the nar operon was no longer repressed by oxygen. The nar d mutation, located upstream of the nar structural genes, was found to be cis dominant; it led to independence from the Fnr protein which, in the wild-type strain, exerts a strict positive control on the nar operon. Both other known controls, nitrate induction and autoregulation, were unaffected. It is proposed that molecular oxygen controls the expression of nar via Fnr and that the nar d mutation affects the Fnr binding site of the narGHI control region.This publication has 36 references indexed in Scilit:
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