Isolation and characterization of a phosphoprotein phosphatase type 2A gene from alfalfa
- 1 July 1993
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 240 (1) , 126-131
- https://doi.org/10.1007/bf00276891
Abstract
Phosphoprotein phosphatases are central regulatory components of the cell cycle in eukaryotes. We report the cloning and sequencing of an alfalfa phosphoprotein phosphatase type 2A (pp2aMs) cDNA. The predicted protein sequence shows high similarity to PP2A from Brassica napus, rabbit and Drosophila. No changes in pp2aMs mRNA abundance during the cell cycle were found. During growth of a batch cell culture, mRNA levels decreased gradually. In planta, all organs contained pp2a transcripts but maximal mRNA levels were detected in stems. Since Southern analysis indicated the presence of a small pp2a gene family in alfalfa, it appears that different subtypes may have specialized roles in various tissues and developmental situations which await characterization.Keywords
This publication has 31 references indexed in Scilit:
- Coupling of mitosis to the completion of S phase in Xenopus occurs via modulation of the tyrosine kinase that phosphorylates p34cdc2Cell, 1992
- Cycling plant cellsThe Plant Journal, 1991
- Identification by molecular cloning of two cDNA sequences from the plant Brassica napus which are very similar to mammalian protein phosphatases‐1 and ‐2AFEBS Letters, 1990
- One of the protein phosphatase 1 isoenzymes in Drosophila is essential for mitosisCell, 1990
- Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycleCell, 1990
- Universal control mechanism regulating onset of M-phaseNature, 1990
- Okadaic acid: a new probe for the study of cellular regulationTrends in Biochemical Sciences, 1990
- Dephosphorylation and activation of Xenopusp34cdc2 protein kinase during the cell cycleNature, 1989
- The bimG gene of Aspergillus nidulans, required for completion of anaphase, encodes a homolog of mammalian phosphoprotein phosphatase 1Cell, 1989
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962