piggyBac‐mediated somatic transformation of the two‐spotted cricket, Gryllus bimaculatus
- 1 August 2004
- journal article
- Published by Wiley in Development, Growth & Differentiation
- Vol. 46 (4) , 343-349
- https://doi.org/10.1111/j.1440-169x.2004.00751.x
Abstract
Transgenic insects have been artificially produced to study functions of interesting developmental genes, using insect transposons such as piggyBac. In the case of the cricket, however, transgenic animals have not yet been successfully artificially produced. In the present study, we examined whether the piggyBac transposon functions as a tool for gene delivery in embryos of Gryllus bimaculatus. We used either a piggyBac helper plasmid or a helper RNA synthesized in vitro as a transposase source. An excision assay revealed that the helper RNA was more effective in early Gryllus eggs to transpose a marker gene of eGFP than the helper plasmid containing the piggyBac transposase gene driven by the G. bimaculatus actin3/4 promoter. Further, only when the helper RNA was used, somatic transformation of the embryo with the eGFP gene was observed. These results suggest that the piggyBac system with the helper RNA may be effective for making transgenic crickets.Keywords
This publication has 18 references indexed in Scilit:
- Involvement of Wingless/Armadillo signaling in the posterior sequential segmentation in the cricket, Gryllus bimaculatus (Orthoptera), as revealed by RNAi analysisMechanisms of Development, 2004
- Hypotonic buffer induces meiosis and formation of anucleate cytoplasmic islands in the egg of the two‐spotted cricket Gryllus bimaculatusDevelopment, Growth & Differentiation, 2003
- Extrachromosomal transposition of the transposable element Minos in embryos of the cricket Gryllus bimaculatusDevelopment, Growth & Differentiation, 2002
- Use of the piggyBac transposon for germ-line transformation of insectsInsect Biochemistry and Molecular Biology, 2002
- Short, Long, and Beyond: Molecular and Embryological Approaches to Insect SegmentationAnnual Review of Entomology, 2002
- 3×P3-EGFP marker facilitates screening for transgenic silkworm Bombyx mori L. from the embryonic stage onwardsInsect Biochemistry and Molecular Biology, 2001
- Germline transformation of Drosophila melanogaster with the piggyBac transposon vectorInsect Molecular Biology, 1999
- Correlation betweenDistal-lessExpression Patterns and Structures of Appendages in Development of the Two-Spotted Cricket, Gryllus bimaculatusZoological Science, 1997
- Using electroporation and a slot cuvette to deliver plasmid DNA to insect embryosGenetic Analysis: Biomolecular Engineering, 1996
- Transposon mutagenesis of baculoviruses: Analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis virusesVirology, 1989