Rapid bioluminescent assay of serum amikacin

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Abstract
The dose-dependent effect of amikacin on accumulation of extracellular bacterial ATP was utilized to develop a rapid bioluminescent assay of serum amikacin. The ATP levels were determined with the firefly bioluminescence system. The accuracy of the bioluminescent assay of amikacin over the therapeutic range expressed as coefficent of variation varied between 1·7–4·6% compared to 1·1–5·8% for an agar disk diffusion assay. Ninety-one serum specimens were assayed with both methods and results were compared (r = 0·97) The bioluminescent assay of amikacin requires only 25 μl of serum and results are available within 90 min.