Nucleotide sequence of bacteriophage G4 DNA

Publisher Website
Google Scholar
Abstract
The 5577 nucleotide long sequence of bacteriophage G4 DNA was determined using the plus and minus and chain termination methods of DNA sequencing. This sequence was compared with that of the closely related bacteriophage .PHI.X174. In the coding regions there is an average of 33.1% nucleotide sequence differences between the 2 genomes, but the distribution of these changes is not random and the sequence of some genes is more conserved than others. There is less sequence similarity between the untranslated intergenic regions of G4 and .PHI.X174, but despite this the sequences of the J/F, F/G and H/A untranslated spaces in both genomes have similar sized hairpin loops, which may be related to their function.