DNA structure, and hemagglutination properties of bovine adenovirus type 2 strains which bypass species specificity

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Abstract
Bovine adenovirus type 2 (Ad bos 2) strains were examined which had been isolated during natural outbreaks among calves, and lambs in Hungary [Belák, S., Pálfi, V.: Arch. ges. Virusforsch.46, 366–369 (1974)]. Differences were detected in hemagglutination properties, and in the restriction site maps of the DNA, which seem to be sufficient to group isolates of Ad bos 2 into two subtypes (subspecies). Some of the strains, recovered from cattle including prototype strain No. 19 are suggested to be separated assubtype A. These viruses hemagglutinate bovine red blood cells, and the physical map of the DNA is similar to, or identical with that of the prototype strain. Virus strains tentatively grouped intosubtype B are pathogenic for both cattle, and sheep under natural conditions. Members ofsubtype B hemagglutinate only rat erythrocytes, and characteristic differences may be detected withBamHI,EcoRI,KpnI, andSalI restriction endonucleases in comparison to the physical maps of the DNA of prototype virus. The genome size of all isolates tested was measured to be ofM r 19.5 to 20.0×106, similar to Ad ovi 1, 4, and Ad bos 4, and 6. All isolates ofsubtype B characteristics were shown to encapsidate heterogeneous genome populations which could be distinguished from those ofsubtype A by the presence of specific restriction endonuclease cleavage fragments with molar ratios of less than 1.0.