Staphylococcus aureus Biofilm Metabolism and the Influence of Arginine on Polysaccharide Intercellular Adhesin Synthesis, Biofilm Formation, and Pathogenesis
- 1 September 2007
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 75 (9) , 4219-4226
- https://doi.org/10.1128/iai.00509-07
Abstract
Staphylococcus aureus and Staphylococcus epidermidis are the leading causes of nosocomial infections in the United States and often are associated with biofilms attached to indwelling medical devices. Despite the importance of biofilms, there is very little consensus about the metabolic requirements of S. aureus during biofilm growth. To assess the metabolic requirements of S. aureus growing in a biofilm, we grew USA200 and USA300 clonal types in biofilm flow cells and measured the extraction and accumulation of metabolites. In spite of the genetic differences, both clonal types extracted glucose and accumulated lactate, acetate, formate, and acetoin, suggesting that glucose was catabolized to pyruvate that was then catabolized via the lactate dehydrogenase, pyruvate formate-lyase, and butanediol pathways. Additionally, both clonal types selectively extracted the same six amino acids (serine, proline, arginine, glutamine, glycine, and threonine) from the culture medium. These data and recent speculation about the importance of arginine in biofilm growth and the function of arginine deiminase in USA300 clones led us to genetically inactivate the sole copy of the arginine deiminase operon by deleting the arginine/ornithine antiporter gene ( arcD ) in the USA200 clonal type and to assess the effect on biofilm development and pathogenesis. Although inactivation of arcD did completely inhibit arginine transport and did reduce polysaccharide intercellular adhesin accumulation, arcD mutants formed biofilms and achieved cell densities in catheter infection studies that were equivalent to those for isogenic wild-type strains.Keywords
This publication has 74 references indexed in Scilit:
- Epidemiologic Distribution of the Arginine Catabolic Mobile Element among Selected Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus IsolatesJournal of Clinical Microbiology, 2007
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Spx Is a Global Effector Impacting Stress Tolerance and Biofilm Formation inStaphylococcus aureusJournal of Bacteriology, 2006
- Genomewide Analysis of Gene Expression inStaphylococcus epidermidisBiofilms: Insights into the Pathophysiology ofS. epidermidisBiofilms and the Role of Phenol‐Soluble Modulins in Formation of BiofilmsThe Journal of Infectious Diseases, 2005
- Low-proline environments impair growth, proline transport and in vivo survival of Staphylococcus aureus strain-specific putP mutantsMicrobiology, 2004
- Microarray Transcription Analysis of Clinical Staphylococcus aureus Isolates Resistant to VancomycinJournal of Bacteriology, 2003
- Impact of theagrQuorum‐Sensing System on Adherence to Polystyrene inStaphylococcus aureusThe Journal of Infectious Diseases, 2000
- Gene replacement in Staphylococcus carnosus and Staphylococcus xylosusFEMS Microbiology Letters, 1997
- Identification of genes involved in utilization of acetate and acetoin in Bacillus subtilisMolecular Microbiology, 1993
- The Effect of Growth Conditions on Oxidative and Dehydrogenase Activity in Staphylococcus aureusJournal of General Microbiology, 1962