Murine Vκ25 Isotype Sequence: Monoclonal Antibody 2S1.3 Specific for the Group A Streptococcal Polysaccharide

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Abstract
Antibodies 2S1.3 and 2S1.1 are hybridoma derived .gamma.3.kappa. murine Ig with specificity for N-acetylglucosamine .beta.1 .fwdarw. 3-linked to L-rhamnose, the immunodeterminant of the streptococcal group A polysaccharide. The [L chain variable region] VL 2S1.3 amino acid sequence is the second complete one reported with this specificity, and it is the first fully determined V.kappa. 25 structure. The [variable kappa] V.kappa. regions of this and the previously described monoclonal anti-A-CHO antibody 7S34.1 are compared with 17 selected partial and complete murine Vk regions, preferentially from antipolysaccharide antibodies. Both V.kappa. 2S1.3 and 7S34.1 increase the variability of existing murine V.kappa. regions; they are, as anti-A-CHO antibodies, most homologous to each other sharing the so far unique residue argine 74. The amino acid sequence of the constant region of 2S1.3 L chain was also determined. It was found to be identical with that of MOPC21, but different from MOPC41 and MOPC70 in residue positions 127, 129, 163 and 166.

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