Reaction of Nitrosoguanidine ( N -Methyl- N ′-Nitro- N -Nitrosoguanidine) with Tobacco Mosaic Virus and Its RNA
- 14 June 1968
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 160 (3833) , 1235-1237
- https://doi.org/10.1126/science.160.3833.1235
Abstract
"Nitrosoguanidine" (N-methyl-N'-nitro-N-nitrosoguanidine) acts on polynucleotides in neutral aqueous solution, methylating guanine in the 7-position, and, to a much lesser extent, adenine. In 67 percent dimethylformamide the effect on the purines decreased, and what seems to be 3-methyl-cytosine appeared. In formamide solution no base changes were detected. Nitrosoguanidine had little mutagenic effect on tobacco mosaic virus RNA in water; it had greater effect in dimethylformamide, and even greater in formamide; it was a strong mutagen only when it acted on intact tobacco mosaic virus particles. Thus, neither the methylation of guanine nor the cytosine modification represents the main mutagenic event.Keywords
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