Modified Enzymatic Methods for the Determination of L-(+)-Lactic and Pyruvic Acids in Blood
- 1 February 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 55 (2) , 152-158
- https://doi.org/10.1093/ajcp/55.2.152
Abstract
Modifications of the enzymatic methods of analyses for L-(+)-lactic and pyruvic acids in whole blood result in average recoveries of 99% for lactate and 100% for pyruvate. The modified lactate procedure facilitates the handling of a large number of analyses. The pyruvate blood recovery studies demonstrated that proper centrifugation following deproteinization is a prime determinant for achieving 100% recovery from whole blood. The importance of technic in performing recovery experiments is emphasized.Keywords
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