A rapid chromatographic method for the determination of ω-N-methylarginines in protein and muscle tissues

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Abstract
A rapid and sensitive chromatographic method is described for the complete separation and determination of NG-methylarginine, NG, NG-dimethylarginine, NG, N''G-dimethylarginine and related compounds in protein and muscle tissue hydrolysates. This method is designed to be used both with conventional amino acid analyzers using a single column (10 .times. 0.9 cm) of Durrum type DC-6A resin at 51.degree. C, 1 buffer system (0.21 M sodium citrate pH 5.40) and 1 buffer flow rate (70 ml/h) and with the accelerated and fully automatic Beckman Spinco model 121M amino acid analyzer using a 10 .times. 0.28 cm microcolumn packed with the Beckman type AA-10 resin and operated with the same buffer at 7.9 ml/h. This procedure was successfully applied to the determination of .omega.-N-methylarginines in various animal tissues [bovine testis, brain, liver and diaphragm], and the presence of 4 as yet unidentified ninhydrin-positive compounds is reported.