Testicular Damage Resulting from Actinomycin D, X-Radiation, and Their Combination in CAF Mice

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Abstract
When the posterior one-half of CAF mice is exposed to x-radiation at 300 or 600 r, and this is combined with actinomycin D at either 75 or 150 [mu]g/kg, administered i.p. x 1, there is significant augmentation of the injury over exposure to one modality alone. This may be measured by increased numbers of cross-sections of seminiferous tubules as viewed in one low-power field, diminished numbers of cell-layers per tubule, and decreased width of tubule. Augmentation of histological abnormalities also occurs, but testicular weights vary only slightly. These parameters are therefore more sensitive indicators of testicular damage than weights. These findings corroborate previous studies that have shown an increase in damage produced by x-ray to normal and neoplastic tissues, when actinomycin D is added.