Tannins in Artemisia montana, A.princeps and Related Species of Plant

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Abstract
The active principles of Artemisia montana and A. princeps as "tannin" of these species were isolated and identified as 3,5-, 3,4- and 4,5-di-O-caffeoylquinic acids. The results of relative astringency based on geraniin (RAG) and relative affinity to methylene blue based on geranin (RMBG) value determination, quantitation of these compounds by high performance liquid chromatography and two-dimensional thin layer chromatography showed that the activity of the extracts of these species are mainly due to these components. Although a comparatively small amount of chlorogenic acid was also isolated, the low values of RAG and RMBG indicate that it is almost indifferent to the activity as "tannin" of these species. Farily abundant presence of these caffeetannis was also found in the hair (moxa) on the leaf of A. montana. The distribution of these caffeetannins in the other species of Artemisia and in the species of several Genera in Compositae, showed that the species which have been used as hemostatics generally contain caffeetannin of composition similarly to those of A. montana and A. princeps and markedly different from that in coffee.