“Radioresistant” intrathymic T cell precursors express T cell receptor Cγ4‐ and Cδ‐specific gene messages
- 1 June 1988
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 18 (6) , 841-847
- https://doi.org/10.1002/eji.1830180603
Abstract
We have studied the expression and sequences of T cell receptor γ and δ chain gene messages in intrathymic T cell precursors of mice irradiated with 600 rads. On day 7 after irradiation a high level of expression of γ and δ chain messages was detected in thymocytes which were composed of a relatively high proportion of CD3+CD4−CD8-thymocytes. During further development of the precursors from day 7 to day 14 after irradiation, γ and δ chain messages fell to low levels and α and β mRNA levels increased. Nucleotide sequence analysis of 14 γ and 10 δ chain complementary DNA (cDNA) in the thymocytes on day 7 revealed that there were 7 functional γ chain transcripts composed of Vγ2-Jγ2-Cγ2 or Vγ1-Jγ4-gene segments, and only 1 functional δ chain transcript composed of the VδM23-Dδ1-Dδ2-Jδl-Cδ gene segments. The repertoire of γ chain and δ chain genes used in “radioresistant” intrathymic T cell precursors of adult mice appears to be limited.This publication has 41 references indexed in Scilit:
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