THE MDG1 MOBILE ELEMENT OF DROSOPHILA CONTAINS 2 REGIONS SPECIFICALLY RECOGNIZED BY PROTEINS IN CRUDE NUCLEAR EXTRACTS

  • 1 December 1989
    • journal article
    • research article
    • Vol. 25  (12) , 2101-2110
Abstract
Two regions in mdg1 mobile element''s body can specifically bind nuclear proteins of Drosophila melanogaster, as demonstrated by the method of retention of DNA-protein complexes of nitrocellulose filters. The first region is situated in the 5''-end part of mdg1, 1 kb downstream the site of initiation of transcription and contains long oligo(A) blocks (from 14 to 30 nucleotides) in the coding chain. The second region is localized near the 3''-LTR and consists of tandem 14-nucleotide repeats and a palindrome, destruction of which leads to weaker binding. There is no competition between the two regions for proteins, which evidence that they are recognized by the different proteins. The binding with the first region can be suppressed by adding the 412 mobile element DNA. These regions are supposed to take place in the regulation of mdg1 transcription.