THE EFFECT OF A SINGLE LARGE DOSE OF 9α-FLUOROPREDNISOLONE ON NUCLEODEPOLYMERASE ACTIVITY AND NUCLEIC ACID CONTENT OF THE RAT THYMUS

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Abstract
The injection of male rats with 25 mg 9a-fluoroprednisolone/kg body weight caused a 29% decrease in thymus RNA concentration 20 hr, after injection. At this time, acid RNase activity had increased to more than twice control values. Alkaline RNase activity in whole thymus homogenates were increased 4 hr. post injection. The removal of an alkaline RNase inhibitor from thymus homogenates greatly altered the response to steroid. Control values of alkaline RNase activity were 6 fold greater in homogenates from which the inhibitor had been removed. No effect of steroid on alkaline RNase activity was detected in homogenates from which the inhibitor was removed. DNase II activity was significantly elevated 2 hr. post injection and reached a peak 2 hr later. No decrease in DNA concentration of thymus homogenates could be demonstrated.