Purification and Characterization of the Messenger RNA Coding for Bovine Corticotropin/β‐Lipotropin Precursor

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Abstract
The mRNA coding for the common precursor of corticotropin and β‐lipotropin has been purified to homogeneity from neurointermediate lobes of bovine pituitaries. The homogeneity of the mRNA preparation is evidenced by analysis of its translation product, electrophoresis on polyacrylamide gel in the presence of formamide and analysis of the kinetics of hybridization with its cDNA. The purification procedure involves the isolation of RNA from membrane‐bound polysomes, chromatography on oligo(dT)‐cellulose and on poly(U)‐Sepharose and sucrose density gradient centrifugation. The mRNA has a molecular weight of approximately 450000, equivalent to approximately 1360 nucleotides in length, and contains a polyadenylate sequence with an average length of 68 nucleotides. The size of the mRNA is sufficiently large to encode the corticotropin/β‐lipotropin precursor.