Enumeration of Indicator Organisms in Foods Using the Automated Hydrophobic Grid-Membrane Filter Technique

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Abstract
The automated HGMF technique was compared against accepted traditional methodology for the recovery and enumeration of coliforms, Escherichia coli, enterococci and Staphylococcus aureus from a variety of naturally and artificially contaminated foods. The overall ratios of recovery of the HGMF relative to conventional methods were 0.88 for coliforms, 0.80 for E. coli, 0.81 for enterococci and 0.80 for S. aureus. Our results suggest that the automated HGMF system is a viable alternative to conventional most-probable-number and spread plate techniques for the isolation and enumeration of foodborne microorganisms on selective media; however, consideration must be given to modifying procedures for the optimal recovery of stressed cells by this automated membrane filtration technique.

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