NAAG Reduces NMDA Receptor Current in CA1 Hippocampal Pyramidal Neurons of Acute Slices and Dissociated Neurons
- 8 September 2004
- journal article
- research article
- Published by Springer Nature in Neuropsychopharmacology
- Vol. 30 (1) , 7-16
- https://doi.org/10.1038/sj.npp.1300559
Abstract
N-Acetylaspartylglutamate (NAAG) is an abundant neuropeptide in the nervous system, yet its functions are not well understood. Pyramidal neurons of the CA1 sector of acutely prepared hippocampal slices were recorded using the whole-cell patch-clamp technique. At low concentrations (20 μM), NAAG reduced isolated N-methyl-D-aspartate receptor (NMDAR)-mediated synaptic currents or NMDA-induced currents. The NAAG-induced change in the NMDA concentration/response curve suggested that the antagonism was not competitive. However, the NAAG-induced change in the concentration/response curve for the NMDAR co-agonist, glycine, indicated that glycine can overcome the NAAG antagonism. The antagonism of the NMDAR induced by NAAG was still observed in the presence of LY-341495, a potent and selective mGluR3 antagonist. Moreover, in dissociated pyramidal neurons of the CA1 region, NAAG also reduced the NMDA current and this effect was reversed by glycine. These results suggest that NAAG reduces the NMDA currents in hippocampal CA1 pyramidal neurons.Keywords
This publication has 50 references indexed in Scilit:
- The three-dimensional organization of the hippocampal formation: A review of anatomical dataPublished by Elsevier ,2003
- Human serine racemase: moleular cloning, genomic organization and functional analysisGene, 2000
- Glutamate carboxypeptidase II is expressed by astrocytes in the adult rat nervous systemJournal of Comparative Neurology, 1999
- Modulation of N -methyl- d -aspartate receptor function by glycine transportProceedings of the National Academy of Sciences, 1998
- The Nagging Question of the Function ofN-AcetylaspartylglutamateNeurobiology of Disease, 1997
- Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase.Proceedings of the National Academy of Sciences, 1996
- N-Acetylated alpha-linked acidic dipeptidase may be involved in axon-Schwann cell signallingJournal of Neurocytology, 1996
- N-acetylated alpha-linked acidic dipeptidase is expressed by non-myelinating Schwann cells in the peripheral nervous systemJournal of Neurocytology, 1995
- The immunocytochemical localization of N-acetylaspartyl glutamate, its hydrolysing enzyme NAALADase, and the NMDAR-1 receptor at a vertebrate neuromuscular junctionNeuroscience, 1995
- Whole cell recording from neurons in slices of reptilian and mammalian cerebral cortexJournal of Neuroscience Methods, 1989