Plant defense in the absence of jasmonic acid: The role of cyclopentenones
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Open Access
- 9 October 2001
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 98 (22) , 12837-12842
- https://doi.org/10.1073/pnas.211311098
Abstract
The Arabidopsis opr3 mutant is defective in the isoform of 12-oxo-phytodienoate (OPDA) reductase required for jasmonic acid (JA) biosynthesis. Oxylipin signatures of wounded opr3 leaves revealed the absence of detectable 3R,7S-JA as well as altered levels of its cyclopentenone precursors OPDA and dinor OPDA. In contrast to JA-insensitive coi1 plants and to the fad3 fad7 fad8 mutant lacking the fatty acid precursors of JA synthesis, opr3 plants exhibited strong resistance to the dipteran Bradysia impatiens and the fungus Alternaria brassicicola. Analysis of transcript profiles in opr3 showed the wound induction of genes previously known to be JA-dependent, suggesting that cyclopentenones could fulfill some JA roles in vivo. Treating opr3 plants with exogenous OPDA powerfully up-regulated several genes and disclosed two distinct downstream signal pathways, one through COI1, the other via an electrophile effect of the cyclopentenones. We conclude that the jasmonate family cyclopentenone OPDA (most likely together with dinor OPDA) regulates gene expression in concert with JA to fine-tune the expression of defense genes. More generally, resistance to insect and fungal attack can be observed in the absence of JA.Keywords
This publication has 39 references indexed in Scilit:
- ORCA3, a Jasmonate-Responsive Transcriptional Regulator of Plant Primary and Secondary MetabolismScience, 2000
- The Arabidopsis DELAYED DEHISCENCE1 Gene Encodes an Enzyme in the Jasmonic Acid Synthesis PathwayPlant Cell, 2000
- Differential Gene Expression in Response to Mechanical Wounding and Insect Feeding in ArabidopsisPlant Cell, 2000
- Octadecanoid-Derived Alteration of Gene Expression and the “Oxylipin Signature” in Stressed Barley Leaves. Implications for Different Signaling PathwaysPlant Physiology, 2000
- Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots.Plant Cell, 1997
- The Critical Requirement for Linolenic Acid Is Pollen Development, Not Photosynthesis, in an Arabidopsis MutantPlant Cell, 1996
- The Pseudomonas phytotoxin coronatine mimics octadecanoid signalling molecules of higher plantsFEBS Letters, 1994
- Arabidopsis Mutants Selected for Resistance to the Phytotoxin Coronatine Are Male Sterile, Insensitive to Methyl Jasmonate, and Resistant to a Bacterial PathogenPlant Cell, 1994
- Arabidopsis Mutants Selected for Resistance to the Phytotoxin Coronatine Are Male Sterile, Insensitive to Methyl Jasmonate, and Resistant to a Bacterial Pathogen.Plant Cell, 1994
- The jasmonate precursor, 12‐oxo‐phytodienoic acid. Induces phytoalexin synthesis in Petroselinum crispum cell culturesFEBS Letters, 1992