The synthesis of L-serine by Micrococcus lysodeikticus

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Abstract
The phosphorylated pathway was established as a biosynthetic route to L-serine in Micrococcus lysodeikticus. Evidence was based upon the demonstration of the synthesis of L-phosphoserine (PS) and serine from D-3-phosphoglyceric acid (PGA) and by the detection of phosphoglyceric acid dehydrogenase and PS phosphatase activities in crude cell-free extracts. The synthesis of PS from PGA was enhanced by L-glutamic acid and by L-lactic dehydrogenase. PS phosphatase was stimulated by added Mg2+ ion. The isolation of ser/gly− auxotrophs lacking PS phosphatase or the ability to synthesize PS from PGA confirmed the obligatory role of the phosphorylated pathway in serine biosynthesis in the organism.

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