EFFECTS OF DIALYZABLE LEUKOCYTE EXTRACTS WITH TRANSFER-FACTOR ACTIVITY ON LEUKOCYTE MIGRATION INVITRO .1. ANTIGEN-DEPENDENT INHIBITION AND ANTIGEN-INDEPENDENT INHIBITION AND ENHANCEMENT OF MIGRATION

  • 1 January 1979
    • journal article
    • research article
    • Vol. 93  (5) , 800-818
Abstract
The effects of DLE [dialyzable leukocyte extracts] containing TFd [dialyzable transfer factor] activity from immune human donors on PBL [peripheral blood leukocytes], obtained from individuals nonresponsive to PPD [purified protein derivative] or Cocci antigen, were evaluated in vitro by the agarose LMI [leukoycyte migration inhibition] technique. Several different preparations of DLE were employed to evaluate the specificity and reproducibility of the effects: from donors skin test positive to PPD but negative to Cocci, from donors skin test negative to PPD but positive to Cocci, from donors skin test positive to both antigens, and from donors skin test negative to both antigens. With PBL from other human donors used as target cells in the direct agarose LMI technique, 3 types of effects were demonstrated for all preparations of DLE: antigen-dependent specific LMI, antigen-independent or nonspecific LMI, and antigen-independent enhancement of migration. The demonstration of each activity depended on the concentration of DLE used and the time allowed for migration. In experiments employing purified PMN [polymorphonuclear neutrophils] and MNL [mononuclear leukocyte] as target cells and a 2-step indirect LMl assay, the antigen-independent effects resulted from the direct action of components in DLE on PMN. The antigen-independent inhibition did not result from toxic effects of DLE. It was produced by DLE but not by dialyzable liver or skin extracts when tested using an amount equivalent to DLE as judged by the absorbance at 260 and 280 nm. The antigen-dependent LMI required secretion of a soluble mediator of MW near 69,000 believed to be LMI. The agarose LMI technqiue is apparently a useful in vitro assay for studies of the mechanism of action of components in DLE which can specifically convert nonimmune lymphocytes to a measurable antigen-sensitive state (i.e., transfer factor). The antigen-independent effects of DLE may be responsible in part for previously reported nonspecific beneficial effects of DLE when used in immunotherapy.

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