Sulfonate analogs of adenosine nucleotides as inhibitors of nucleotide-binding enzymes

Abstract
2-(Adenin-9-yl)ethanesulfonic acid (1), 3-(adenin-9-yl)propanesulfonic acid (2), 9-(5-deoxy-.beta.-D-ribofuranosyl)-adenine-5''-sulfonic acid (3) and 9-(3-deoxy-.beta.-D-arabinofuranosyl)adenine-3''-sulfonic acid (4) were prepared by reaction of the corresponding chlorides by sodium sulfite (1-3) or by reaction of an epoxide with sodium hydrogen sulfite (4). They inhibited a typical nucleotide-binding enzyme, horse liver alcohol dehydrogenase, with inhibition constants in the range of 0.18-4.9 mM at pH 8 and 25.degree. C.