Plasmid maintenance in Bacillus subtilis recombination-deficient mutants
- 1 June 1987
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 208 (1-2) , 349-352
- https://doi.org/10.1007/bf00330464
Abstract
An isogenic set of 11 recombination-deficient mutant strains of Bacillus subtilis has been constructed. Whereas plasmid pUB110 is stably maintained in such Rec- cells, the high copy number plasmid pC194 is unstable. Instability in Rec- strains could be mostly attributed to the deleterious effect of the presence of the plasmid on the Rec- cells' growth capability. In part, instability of pC194 derivatives could also be correlated with the presence of an unusually high amount of multimeric DNA molecules.Keywords
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