ON THE BIOSYNTHESIS AND STRUCTURE OF DOUBLE-STRANDED RNA IN VACCINIA VIRUS-INFECTED CELLS
- 1 September 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 64 (1) , 396-403
- https://doi.org/10.1073/pnas.64.1.396
Abstract
The virus-specific, RNase-resistant RNA appearing in vaccinia virus-infected cells was directly shown to be an RNA duplex. After its melting and subsequent banding on Cs 2 SO 4 or incubation with DNase, the RNA could be reannealed and then hybridized with vaccinia virus DNA. The double-stranded virus-specific RNA appears to exist in the cell in the form of heterogeneous partially double-stranded RNA's sedimenting between 9 and 22 S . The kinetics of the appearance of the double-stranded RNA in the cell show a dependence on the multiplicity of infection and suggest that the double-stranded RNA is a “late” product of viral intracellular biosynthesis. The synthesis of the double-stranded RNA is inhibited by actinomycin D.Keywords
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